Understanding Rosanilin Staining in Histology and Cytopathology

Rosanilin is a type of pigment that is commonly used in histology and cytopathology to stain tissue samples. It is a mixture of two dyes, eosin and carmine, which are combined to create a pink or red color. Rosanilin is often used to highlight the nuclei of cells in tissue samples, as it binds specifically to the DNA and RNA in these structures.

Rosanilin is also known as "eosin-carmine" or "E&C stain." It is a popular stain for use in histology laboratories because it is relatively inexpensive and easy to use, and it provides good contrast between the nuclei of cells and the surrounding tissue. However, it is not as specific as some other stains, such as hematoxylin and eosin (H&E), and it may not be suitable for all types of tissue samples.